Initially, in the natural drinking water, spiked with an input bacterial load of 105 colony forming units (CFU)/mL (typical natural drinking water sources contain a maximum of 102–103 CFU/mL, in rural India), certain quantity of Ag+ (in the range of 10 to 100 ppb) was added separately and the microbial survival was tested after a contact time of 1 h. A concentration of 50 ppb was found to be the active Ag+ input concentration needed for maximum antibacterial activity (up to 99%), in natural drinking water. Even at an enhanced concentration of 1 ppm Ag+, the residual microbial concentration was not diminished. It is important to note that chloride ions invariably present in natural water (1–250 ppm) leads to speciation of Ag+ and thus reduces the availability of silver ions. Silver chloride complexes [AgCl (aq), (AgCl)Ag+, (AgCl)Cl−, (AgCl)n and their aggregates) due to their negative charge are nearly 300 times less in toxicity to bind with bacterial surface when compared to free silver ions13. With increasing concentrations of chloride ions (up to 200 ppm as in natural waters) at a fixed silver ion concentration, the speciation increases leading to a drop in the performance of bactericidal activity. At 200 ppm of Cl−, silver exists as 0.16% of [AgCl3]2−, 31% of [AgCl2]−, 63% of AgCl (aq) and only 5.8% of input silver remains as Ag+ (Supplementary Fig. S1). With Ag+ alone, no significant antiviral activity was observed for MS2 bacteriophage in tap water. Silver in the ionic form continue to be available during the period of antimicrobial activity in the test water conditions (Supplementary Fig. S2).
The residual microbial concentration (to the extent of 1% or 103 CFU/mL of E. coli and essentially all of the input viral load) still present is a challenge for reliable water purification. To address this economically and sustainably, the above method was improved upon. To 50 ppb Ag+, various anions, namely NO3−, SO42−, SiO42−, PO43, I−, Br− and CO32− (or species derived from them, at prevalent conditions), as many of them can also be present in natural waters, were added at varying amounts to achieve a cent percent potential system. Among these, SiO42− and CO32− were promising compared to other anions and the latter was more significant in this property. Therefore, the experiments were designed to reduce the Ag+ concentration to a minimum at acceptable CO32− levels, sufficient to kill bacterial and viral cells in the natural drinking water. It is important to note that antimicrobial activity due to carbonate alone was insignificant even at 100 mM (10,000 ppm) Na2CO3. However, we found that in presence of CO32−, 50% lower concentration of silver i.e., 25 ppb Ag+, was enough to obtain a complete (100,000 times) reduction in the input bacterial load (Supplementary Fig. S3). Therefore, while 50 ppb Ag+ showed a 100 times reduction (from 105 CFU/mL to 103 CFU/mL), a combination of 25 ppb Ag+ + 20 ppm CO32− showed 100,000 times reduction (from 105 CFU/mL to 100 CFU/mL) resulting in 1,000 times enhanced antimicrobial effect.
In order to explain the carbonate's promotional activity, a combined Ag+ (25 ± 10 ppb) and CO32− (20 ± 5 ppm) release device was prepared, using silver nanoparticle (AgNP)-loaded biopolymer composite14 and a sodium carbonate pellet, respectively. The output water from the device was spiked with E. coli and MS2 bacteriophage at required concentrations following which the water was tested for microbial survival (see Methods). In presence of CO32− (~20 ppm) the combination was found to possess a significant antimicrobial activity at concentrations of Ag+ as low as 20 ± 10 ppb within a contact time of 15 min. Thus CO32− not only minimizes the concentration of silver required, but also increases the kinetics of the reaction (Supplementary Fig. S4). The combination was also tested for antibacterial activity against a gram positive organism, Staphylococus aureus at similar bacterial load used for E. coli. Pour plate experiments showed that the antibacterial property of the combination was similar for both gram positive and gram negative bacteria. In order to confirm the bactericidal activity of this combination, the water was given a standing time of 24 h and tested for bacterial viability. The overnight standing experiments showed that the combination acted as bactericidal agent and not as bacteriostatic (Supplementary Fig. S5). The combination was tested for cytotoxicity on human rhabdomyosarcoma RD cells and no such effect was seen.
Considering the fact that the major water purification technique used for virus removal is the addition of chlorine which produces harmful DBPs, we propose that the Ag+ + CO32− combination, in addition could also serve as an alternative to reduce pathogenic viruses by a safer method. Here, we tested the combination for antiviral property on MS2 phage. A sequential time interval study of 50 ppb Ag+ alone and a combination of 50 ppb Ag+ and 20 ppm CO32− was performed (see Methods). We observed that while 50 ppb Ag+ alone (even after 1–2 h contact) was unable to affect the phage, the combination of 50 ppb Ag+ and 20 ppm CO32− was found to achieve effective antiviral property within a contact time of 15 min (Supplementary Fig. S6). Moreover, by this study, we proved that this combination can handle virus at concentrations likely in ground water (103 plaque forming units (PFU)/mL) but can also efficiently control comparatively higher concentrations (~106 PFU/mL) that can prevail in non-portable water (Supplementary Fig. S7).
A defective outer membrane/capsid is suspected as the reason for enhanced activity of the combination. In order to confirm this, we conducted detailed dark field fluorescence imaging of bacteria and transmission electron microscopy of the viruses. Fluorescent staining experiments using dark field microscopy can distinguish between intact and membrane-permeable cells using LIVE/DEAD BaclightTM bacterial viability kit. The two stains used were the membrane impermeant, propidium iodide (PI), causing red fluorescence of membrane permeabilized cells and SYTO 9, a nucleic acid binding green fluorescent dye that could enter into all the cells regardless of whether they were intact or permeabilized. Defective PI fluorescing bacteria were observed as red when they were treated with Ag+ + CO32− whereas untreated and Ag+-treated bacteria (partly) show SYTO 9 emission (green) (Fig. 1 i, ii). Membrane permeabilization is seen in all the bacteria upon treatment with Ag+ + CO32− (Fig. 1 C, F). We note that this happens for both E. coli and S. aureus, showing the effectiveness of the composition for gram negative and gram positive bacteria. Similarly, uranyl acetate stain enters defective viral capsids. Therefore, distinct difference in contrast is observed for carbonate-treated and untreated viruses under transmission electron microscopy (TEM)15. Dense dark centred capsids (defective capsids) which had taken up the uranyl acetate stain were seen in the Ag+ + CO32− treated virus whereas untreated/Ag+ treated viruses were found to be rigid which did not show the stain (Fig. 1 iii). These experiments prove that there is a significant damage to the outer protective layer of the bacterial/viral membrane as a result of Ag+ + CO32− treatment.
Figure 1The fluorescence microscopy and HRTEM images demonstrating the defective outer membrane/capsid in microbes caused due to Ag+ + CO32− treatment.
Line (a): Fluorescence microscopy images of E. coli: ([i] Input bacteria, [ii] 50 ppb Ag+-treated bacteria and [iii] 50 ppb Ag+ + 20 ppm CO32−-treated bacteria after staining with syto9 and propidium iodide. Line (b): Similar fluorescence microscopy images of S. aureus: [iv] Input bacteria, [v] 50 ppb Ag+-treated bacteria and [vi] 50 ppb Ag+ +20 ppm CO32−-treated bacteria. Line (c) TEM images of MS2 bacteriophage: [vii] Input viruses, [viii] 50 ppb Ag+-treated viruses and [ix] 50 ppb Ag+ + 20 ppm CO32−-treated viruses after staining with 0.2% uranyl acetate. Images (vii) and (viii) appear the same as they are not damaged, while image (ix) appears dark as damage in the capsid allowed the stain to pass through it.
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Enhancement that carbonate creates on silver's antimicrobial performance was confirmed by studying the changes in Ag+ uptake by bacteria and viruses, using inductively coupled plasma mass spectrometry (ICP MS). In these experiments, the bacteria and viruses, after treatment with Ag+ and Ag+ + CO32− at various incubation times were separated carefully and the filtrates were analyzed accurately for their silver content. Appropriate blanks were conducted. The bacteria and viruses showed intake of Ag+ up to 90% (±5%) in presence of CO32− within a contact time of 10 min, whereas in its absence, the intake was less than 50% (Fig. 2). ICP MS results showed that the rate of uptake is an order of magnitude larger with carbonate (k(Ag+) = 0.0001, k(Ag++CO3) = 0.001 (both in min−1), assuming a first order process.
Figure 2ICP MS measurement of silver intake by bacteria and viruses.
Concentration of silver in bacteria (a) and viruses (b) upon various treatments. Input concentration of silver is 50 ppb in the input water. Upon incubation with bacteria and viruses, Ag+ concentration in the solution decreases while that in the organisms increase. These are plotted with time of incubation, measured by careful filtration through 100 kDa ultrafiltration membranes. After incubation with Ag+; filtered water (i) and bacteria/viruses (ii). After incubation with Ag+ + CO32−; filtered water (iii) and bacteria/viruses (iv). Blank measurements without bacteria and viruses; filtered water (v); concentration on the membrane (vi). Measurements show that silver ion uptake is nearly quantitative; some loss of Ag+ on the membrane as shown by trace (vi) is unavoidable as all membranes pick a small amount of silver. Sum of (i) and (ii) at 225 min does not give exactly 50 ppb due to this reason.
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Considering that the increase in the pH (from 7.0 to 8.5) due to the addition of CO32− might be the reason behind this enhancement in the antibacterial effect of Ag+, we performed experiments in natural drinking water with an adjusted pH of 8.5 using NaOH. The treated output was examined for microbial viability. Results of several experiments concluded that the NaOH-induced pH change did not enhance the antimicrobial activity of Ag+.
The enhanced effect due to carbonate is suspected to be due to the peripheral proteins of the membrane. These peripheral proteins are associated with the membrane lipids and other proteins via electrostatic forces or hydrophobic interactions. Thus, conditions like high salt concentrations or an alkaline environment disrupt the interactions leading to the detachment of peripheral proteins16. The distinctive property of carbonate in the removal of peripheral membrane proteins of low molecular weight has been reported in animal cells17 and E. coli K-12 cells18. Therefore, in presence of CO32−, a disturbed membrane, free of peripheral proteins is suspected, which allows the penetration/cellular mobility of Ag+, by increasing the bioavailability of silver ions. Fig. 3 is a schematic representation of this likely mechanism. In Fig. 3(i), bacteria are subjected to Ag+ ions at a concentration ≤100 ppb. Ag+ ions bind to negatively charged membrane proteins in a non-selective way and sufficient ions are unavailable to enter into cells to cause cell damage. In Fig. 3(ii), bacteria are subjected to Ag+ at concentrations as low as 25 ppb along with 20 ppm CO32−. In presence of CO32−, several membrane-bound peripheral proteins are removed and thus Ag+ binds directly to the cell surface. As a result, increased number of Ag+ enter into the exposed cells and cause sufficient damage in them.
Figure 3Schematic representation of suspected mechanism behind the unprecedented enhancement of antimicrobial property of silver in presence of carbonate.
(a) Healthy cells treated up to 100 ppb Ag+ showed inadequate cell damage. (b) Healthy cells after exposed to 20 ppm CO32− were damaged completely with 25 ppb Ag+.
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To verify this possibility, the treated bacteria were tested for the presence of low molecular weight membrane proteins/peptides using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) (see Supplementary Note 1). Specific peaks at a set of m/z values (7,000–10,000) were observed in the control and Ag+-treated bacterial cells whereas such peaks were completely absent in the case of carbonate-treated cells (Supplementary Fig. S8). The peaks obtained in the range of m/z 7,000–10,000 correspond to several proteins in E. coli which include both mature and immature proteins and peptides that are released from membrane bound proteins19. It is clear that carbonate, by acting upon membrane proteins/peptides, removes them. Removal of these membrane proteins increases the active-silver (as they offer a barrier for penetration by binding it) that leads to the total bioavailability of silver, in accordance with the uptake studies presented above. Separation of specific proteins responsible for the enhanced activity due to carbonate was difficult due to their reduced concentrations.
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